Immobilized degradable cationic polymer for transfecting...

Details Immobilized degradable cationic polymer for transfecting... Immobilized degradable cationic polymer for transfecting...

C - Chemistry, Metallurgy

12

M

C12M 1/16 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2590820

A cell transfection/culture device is disclosed which includes a solid support coated with a degradable polymer cation as a transfection reagent. The transfection/culture device is conveniently stored at room temperature until use. Cell transfection is accomplished easily by adding the nucleic acid of interest and the cells to be transfected to the transfection/culture device. Cell transfection is completed in less than one hour by using the transfection/culture device described herein.

L'invention concerne un dispositif de transfection/culture cellulaire qui comprend un support solide recouvert d'un cation polymère dégradable en tant que réactif de transfection. Ce dispositif de transfection/culture cellulaire est stocké de manière pratique à température ambiante jusqu'à son utilisation. Pour effectuer facilement la transfection cellulaire, l'acide nucléique d'intérêt et les cellules à transfecter sont ajoutés au dispositif de transfection/culture. La transfection cellulaire est achevée en moins d'une heure au moyen du dispositif de transfection/culture selon l'invention.

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