Immobilized enzymes as biosensors for chemical toxins

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/46 (2006.01) C12Q 1/34 (2006.01) C12Q 1/44 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2372175

Methods, compositions and materials useful in the detection of organophosphorous and organosulfur are disclosed. In particular, biosensors wherein a porous e.g. foam or a non-porous support having an enzyme covalently immobilized upon or within are disclosed. The biosensors exhibit enzymatic stability at extreme temperatures and/or denaturing conditions, and similar kinetic characteristics of the soluble form of the enzymes utilized. The enzyme does not leach from the porous or non-porous support and the material retains enzymatic activity after prolonged storage. Differential biosensors are also disclosed.

L'invention porte sur des procédés, des compositions et des matériaux servant à la détection de composés organophosphoreux et organosulfureux et en particulier sur des biodétecteurs comportant un support poreux ou non sur lequel ou dans lequel une enzyme est immobilisée. Lesdits biodétecteurs présentent une bonne stabilité enzymatique aux températures extrêmes et/ou dans des conditions dénaturantes, et des caractéristiques cinétiques similaires à celles de la forme soluble des enzymes utilisées. Les enzymes ne se délavent pas du support, poreux ou non poreux, et le matériau conserve son activité enzymatique après un stockage prolongé. L'invention porte également sur des biodétecteurs différentiels.

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