C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/00 (2006.01) C10L 1/08 (2006.01) C12N 9/96 (2006.01) C12P 7/64 (2006.01) C12N 9/20 (2006.01)
Patent
CA 2675003
Disclosed is a process for the preparation of an interfacial enzyme immobilized on an insoluble support, by providing a bi-phase system comprised of an aqueous buffer solution and at least one first organic solvent; mixing said interfacial enzyme with the bi-phase system provided; adding the support to the obtained mixture and mixing; and isolating from the mixture obtained in the last step the interfacial enzyme immobilized on said support. The produced enzyme is locked in its catalytically active confirmation, and thus exhibits improved activity and stability. Also disclosed are uses of the produced enzymes, particularly in the preparation of biodiesel.
L'invention concerne un procédé de préparation d'une enzyme interfaciale immobilisée sur un support insoluble qui comprend les étapes consistant à : fournir un système diphasique constitué d'une solution tampon aqueuse et d'au moins un premier solvant organique ; mélanger ladite enzyme interfaciale au système diphasique fourni ; ajouter le support au mélange obtenu et mélanger ; et isoler du mélange obtenu dans la dernière étape l'enzyme interfaciale immobilisée sur ledit support. L'enzyme produite est verrouillée dans sa conformation catalytiquement active, et présente ainsi une activité et une stabilité améliorées. L'invention concerne également des utilisations des enzymes produites, en particulier dans la préparation du biodiesel.
Fasken Martineau Dumoulin Llp
Trans Biodiesel Ltd.
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