Immobilized oligo-nucleotide probes and uses therefor

Details Immobilized oligo-nucleotide probes and uses therefor Immobilized oligo-nucleotide probes and uses therefor

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) B01J 19/00 (2006.01)

Patent

CA 2049043

2049043 9010716 PCTABS00002 The invention is a method of detecting nucleic acids in a sample using oligonucleotide probes which are noncovalently bound to solid supports for rapid, sensitive, hybridization assays. The method involves coating the support surface with a polynucleotide and then hybridizing a specific capture probe for each analyte to the polynucleotide by way of a short tail of the complementary polyunucleotide. The immobilized probes are used to capture nucleic acid targets out of complex specimens for nonisotopic detection without the need for prior cell culture or purification of the target nucleic acids. A panel of tests can be run on each specimen simultaneously, a format that conserves precious samples. The assay can be readily automated, and can be conveniently run in a manual fashion on large numbers of samples in two to three hours.

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