Immobilized sequence-specific probes

Details Immobilized sequence-specific probes Immobilized sequence-specific probes

C - Chemistry, Metallurgy

12

Q

150/3, 195/1.12,

C12Q 1/68 (2006.01)

Patent

CA 1340864

An improved nucleic acid hybridization assay reagent capable of binding a nucleic acid having a selected target sequence comprises a solid support matrix having oligonucleotide probes covalently attached thereto via a spacer arm. In a preferred embodiment, the solid support has reactive amino groups capable of binding UV irradiated nucleotides in an oligonucleotide probe, which probe comprises a hybridizing region complementary to a target sequence to be detected and a linking tail, the tail being composed of nucleotides, one or more of which is covalently bonded to the reactive groups of said support.

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