Immortalisation of mammalian cells and therapeutic...

C - Chemistry – Metallurgy – 07 – K

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C07K 14/025 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2536655

Genomic instability in T-antigen expressing cells can be overcome by modifying the gene expressing T-antigen so that it lacks Bub1 binding. Stable cell lines can be produced by incorporation of the modified T-antigen gene, preferably together with the catalytic sub-unit of the telomerase construct.

L'instabilité génomique dans des cellules exprimant l'antigène T peut être surmontée par modification du gène exprimant l'antigène T de manière qu'il ne puisse pas se lier à Bub1. Des lignées cellulaires stables peuvent être produites par incorporation du gène de l'antigène T modifié, de préférence avec la sous-unité catalytique du produit de recombinaison télomérase.

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