Immortalized avian cell lines and use thereof

A - Human Necessities – 61 – K

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Details

A61K 39/275 (2006.01) A61K 38/18 (2006.01) A61K 39/12 (2006.01) C12N 7/02 (2006.01)

Patent

CA 2761457

The present invention relates to specific immortalized avian cell lines expressing telomerase reverse transcriptase (TERT), and exhibiting distinct biologics production patterns. More particularly, the present invention relates to immortalized avian cell line capable of either amplifying Flaviviridae but not capable of amplifying Vaccinia virus strain Copenhagen(VV-COP) nor Modified Vaccinia virus Ankara (MVA), or capable of amplifying both Flaviviridae and Poxviridae. The invention further relates to the use of said immortalized avian cell lines and related methods for producing biologics, including viruses and proteins.

La présente invention concerne des lignées cellulaires aviaires immortalisées spécifiques exprimant la transcriptase inverse de la télomerase (TERT), et présentant des schémas de production de substances biologiques distincts. Plus particulièrement, la présente invention concerne une lignée cellulaire aviaire immortalisée pouvant soit amplifier Flaviviridae mais ne pouvant amplifier ni la souche de virus de la vaccine Copenhagen (VV-COP) ni le virus modifié de la vaccine Ankara (MVA), ou pouvant amplifier à la fois Flaviviridae et Poxviridae. L'invention concerne en outre l'utilisation desdites cellules aviaires immortalisées et de procédés apparentés pour produire des substances biologiques, notamment des virus et des protéines.

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