Immortalized avian cell lines for virus production

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) C12N 5/071 (2010.01) C12N 7/00 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2544462

The present invention relates to immortalized avian cell lines suitable for production of biologicals or viruses for vaccination. In particular, the cell lines are derived from primary cells which are transformed with at least two viral or cellular genes, one of which causes cell cycle progression whereas the other interferes with innate protective mechanisms of the cell induced by dysregulated replication. The invention moreover relates to the production of said immortalized cell lines and their use for producing biologicals or viruses for vaccination.

Cette invention se rapporte à des lignées de cellules aviaires immortalisées destinées à la production de produits biologiques ou de virus pour la vaccination. Ces lignées de cellules sont notamment dérivées de cellules primaires qui sont transformées par au moins deux gènes viraux ou cellulaires, dont l'un entraîne la progression du cycle cellulaire, alors que l'autre interfère avec les mécanismes de protection innés de la cellule induits par une réplication dérégulée. Cette invention concerne en outre la production de ces lignées de cellules immortalisées et leur utilisation pour produire des produits biologiques ou des virus pour la vaccination.

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