Immortalized human cell lines containing exogenous...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12Q 1/02 (2006.01) C12Q 1/26 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2118043

Non-tumorigenic, human bronchial epithelial cell lines are provided wherein the cell lines are capable of expressing cytochrome P450 genes which have been inserted into the cell lines. Also provided are methods and kits for identifying potential mutagens, cytotoxins, carcinogens, and chemotherapeutic agents utilizing these cell lines.

L'invention concerne des lignées cellulaires épithéliales bronchique humaines, non oncogènes dans lesquelles des lignées cellulaires peuvent exprimer des gènes P450 cytochromes insérés dans les lignées cellulaires. L'invention concerne en outre des procédés et des nécessaires permettant d'identifier les mutagènes, les cytotoxines, les carcinogènes et les agents chimiothérapiques potentiels utilisant ces lignées cellulaires.

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