Immortalized human keratinocyte cell line

Details Immortalized human keratinocyte cell line Immortalized human keratinocyte cell line

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C12N 5/071 (2010.01) C12N 5/09 (2010.01) C12N 5/22 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2336717

A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.

L'invention concerne une lignée cellulaire de kératinocytes humains immortalisés spontanément. Dans un mode de réalisation préféré, cette lignée cellulaire est ATCC 12191. Dans un autre mode de réalisation, elle concerne un procédé permettant de doser l'effet d'un agent d'essai de modulation de cellules cancéreuses. Ce procédé consiste à obtenir une culture de cellules épithéliales squameuses stratifiées humaines contenant des cellules épithéliales squameuses malignes et des kératinocytes humains immortalisés spontanément, cette culture formant un épiderme reconstitué. On traite ensuite l'épiderme au moyen d'un agent d'essai de modulation de cellules cancéreuses et on évalue la croissance des cellules malignes à l'intérieur de l'épiderme.

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