Immortalized vascular pericyte line

Details Immortalized vascular pericyte line Immortalized vascular pericyte line

C - Chemistry, Metallurgy

12

N

C12N 5/16 (2006.01) A01K 67/027 (2006.01) C07K 14/025 (2006.01) C12N 15/18 (2006.01) C12N 15/37 (2006.01) C12N 15/63 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2400361

To provide an immortalized capillary pericyte line which maintains the original function/property of the cell line-deriving tissue, its establishment method, and the screening method for useful substance using the immortalized capillary pericyte line. Cerebral tissue of a transgenic rat carrying the large T antigen gene of SV40 thermo-sensitive mutant line tsA58 is homogenized and the resultant brain capillaries are treated with protease, thus obtained brain capillary cells are subcultured to establish an immortalized cell that expresses SV40 thermo-sensitive large T antigen, PDGF receptor a , and Angiopoietin-1. In addition, the immortalized vascular pericyte line has ability to deposit calcium on matrix by dense culture.

L'invention concerne une lignée cellulaire adventitielle capillaire, immortalisée, possédant les fonctions et caractéristiques inhérentes au tissu duquel elle est dérivée. Elle concerne également un procédé d'établissement de cette lignée, ainsi qu'un procédé de criblage d'une substance utile, mettant en oeuvre cette lignée cellulaire. Selon l'invention, on homogénéise un tissu cérébral d'un rat transgénique possédant le gène de l'antigène grand-T d'un mutant tsA58 thermosensible du virus SV40, transféré dans ce tissu, puis on traite les capillaires ainsi obtenus au moyen d'une protéase. En effectuant une sous-culture de cellules capillaires cérébrales ainsi obtenues, on obtient des cellules immortalisées exprimant l'antigène grand-T du virus SV40 thermosensible, le récepteur beta de PDGF, et l'angiopoiétine-1. De même, cette lignée cellulaire adventitielle vasculaire peut déposer une masse matricielle de calcium.

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