Immunity memory cell suspension and method of preparing same

C - Chemistry – Metallurgy – 12 – N

Patent

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167/114, 195/44,

C12N 5/00 (2006.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01) C12N 5/06 (2006.01)

Patent

CA 1333887

A method of preparing an immunity memory cell suspension, wherein cells consisting of T-cells are separated from cells collected from an immunity sensitized individual immunized against a particular pathogen and are mixed with an antibody reactive to a histocompatibility antigen specific to a pre- scribed species of cells for producing antigen-antibody complexes by the antibody and those of the separated cells which have the histocompatibility antigen, whereupon a blood serum is added to the cells containing the antigen-antibody complexes for causing the complement in the blood serum to bind to the antigen-antibody complexes and thereby destroying the cells having the histocompatibility antigen and allowing the remaining cells to persist. To these persisting cells is then added an antibody reactive to a histocompatibility antigen specific to immunity memory cells and from the resul- tant cells are separated the immunity memory cells which have the histocompatibility antigen specific to immunity memory cells. Immunity memory cells may be cultured and subcultured successively through a plurality of generations to establish a continuous line of cells which originates in the immunity memory cells thus separated from the initial immunity sensi- tized individual.

596787

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