Immunoaffinity purification system

Details Immunoaffinity purification system Immunoaffinity purification system

C - Chemistry, Metallurgy

12

N

195/1.112, 530/3

C12N 15/62 (2006.01) C07K 7/06 (2006.01) C07K 14/535 (2006.01) C07K 14/545 (2006.01) C07K 16/24 (2006.01) C07K 16/44 (2006.01) C12N 5/18 (2006.01) C12P 21/08 (2006.01)

Patent

CA 1307752

IMMUNEX CORPORATION 2203 TITLE Immunoaffinity Purification System ABSTRACT A process is disclosed for purifying a recombinant fusion protein having an N-terminal identification sequence comprising multiple anionic amino acid residues, comprising forming a complex of the protein with a divalent cation dependent monoclonal antibody specific for the sequence, isolating the complex, and dissociating antibody and protein by selectively depleting the concentration of divalent cations in contact with the complex. A particular calcium-dependent monoclonal antibody, 4E11, is useful in an embodiment of the process which employs the identification peptide DYKDDDDK.

554765

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