Immunoassay

G - Physics – 01 – N

Patent

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Details

G01N 33/569 (2006.01) G01N 33/543 (2006.01) G01N 33/558 (2006.01)

Patent

CA 2164316

An improved Western blot method wherein the captured antibody of a test sample is detected by a probe specific for the captured antibody. The capture reagent can be a lysate, a recombinant protein or a synthetic peptide specific for the al tibody to be detected. A test kit for performing the method also is provided.

L'invention concerne une méthode de transfert Western blot, dans laquelle l'anticorps capturé d'un échantillon d'analyse est détecté par une sonde qui est spécifique dudit anticorps. Le réactif de capture peut être un lysat, une protéine de recombinaison ou un peptide synthétique qui sont spécifiques de l'anticorps à détecter. Un matériel d'essai utilisé pour appliquer cette méthode est également décrit.

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