Immunoassay and kit for an early and simultaneous detection...

G - Physics – 01 – N

Patent

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Details

G01N 33/53 (2006.01) G01N 33/533 (2006.01) G01N 33/534 (2006.01) G01N 33/543 (2006.01) G01N 33/573 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2507024

The present invention comprises an immunochemical assay for determination of at least two antigens in a sample. The immunochemical assay comprises contacting a sample from a patient with a carrier molecule that contains at least two capture antibodies, each of which specifically binds to a binding moiety of an antigen in the sample. The assay further contains a detection antibody that specifically binds the same antigens on different binding moieties than binding moieties used by the capture antibodies. The detection agent is further attached to one or more detection probes to facilitate the detection of antigens in the sample. The immunochemical assay of the present invention is specifically designed to detect biochemical markers that are released at different time intervals in a patient's sample.

La présente invention concerne un dosage immunochimique permettant de détecter au moins deux antigènes dans un échantillon. Le dosage immunochimique comprend la mise en contact d'un échantillon d'un patient avec une molécule porteuse contenant au moins deux anticorps de capture, se fixant chacun de manière spécifique à un fragment de liaison d'un antigène dans l'échantillon. Le dosage contient également un anticorps de détection se fixant de manière spécifique aux mêmes antigènes sur différents fragments de liaison que les fragments de liaison utilisés par les anticorps de capture. L'agent de détection est également connecté à une ou plusieurs sondes de détection afin de faciliter la détection des antigènes dans l'échantillon. La dosage immunochimique de la présente invention est spécifiquement destiné à détecter des marqueurs biochimiques libérés à différents intervalles de temps dans l'échantillon d'un patient.

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