G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2224674
The present invention relates to an immunological test kit and immunoassay using a first immobilized antibody having affinity for a specific antigen. The invention is characterized by a second and third antibody being specific for different determinants of the antigen and modified with cross-linkable oligonucleotides. For detection, the oligonucleotides are amplified, whereby only such oligonucleotides will be amplified which have been cross-linked to each other. In this way unspecific background is avoided and detection is possible down to single molecules.
La présente invention se rapporte à un kit pour essais immunologiques dans lesquels on utilise un premier anticorps immobilisé ayant une affinité pour un antigène spécifique. L'invention est caractérisée par un second et un troisième anticorps, spécifiques de différents déterminants de l'antigène et modifiés par des oligonucléotides réticulables. Aux fins de détection, on amplifie uniquement les oligonucléotides qui ont été réticulés entre eux. De cette manière, on évite tout contexte non spécifique et l'on rend la détection possible jusqu'au niveau de simples molécules.
Landegren Ulf
Olink Ab
Smart & Biggar
LandOfFree
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