Immunoassay apparatus

Details Immunoassay apparatus Immunoassay apparatus

G - Physics

01

N

167/43

G01N 21/64 (2006.01) G01N 21/77 (2006.01)

Patent

CA 1266826

ABSTRACT An assay apparatus employing total internal reflection of excitation radiation at the interface between an optically conductive rod or fiber and a surrounding liquid phase of lower index of refraction. Immobilized on the surface of the fiber is a component of a complex formed in an immunochemical- type reaction; a fluorophore that can be excited into fluorescence by the excitation radiation is attached to another component of the complex. The fiber is coaxially disposed in cantilevered manner within a length of tubing, so that the excitation radiation can be launched into the unsup- ported end of the fiber and the fluorescent radiation tun- neling back into the fiber may be observed at the same fiber end, thereby preventing that portion of the fiber surface bet- ween the unsupported fiber end and the mounted end being in contact with other than the surrounding liquid phase.

517705

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