Immunoassay employing two-step internal calibration reaction

Details Immunoassay employing two-step internal calibration reaction Immunoassay employing two-step internal calibration reaction

G - Physics

01

N

G01N 33/543 (2006.01)

Patent

CA 2588230

Methods for quantitatively measuring the amount of an analyte of interest in a fluid sample, and kits useful in the methods, are disclosed. The methods comprise sandwich assays, and utilize an internal calibration reaction that closely mimics the reaction of test particles by the use of a two-step reaction.

L'invention concerne des procédés permettant de mesurer sur le plan quantitatif la quantité d'un analyte étudié dans un échantillon de fluide et des kits utiles dans les procédés. Les procédés mettent en oeuvre des dosages sandwich et une réaction d'étalonnage interne imitant de près la réaction de particules test, au moyen d'une réaction en deux étapes.

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