Immunoassay for biologically active human interferon- gamma...

Details Immunoassay for biologically active human interferon- gamma... Immunoassay for biologically active human interferon- gamma...

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167/139, 167/41,

G01N 33/577 (2006.01) C07K 14/57 (2006.01) C07K 16/24 (2006.01) C12N 15/00 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1242658

-57- IMMUNOASSAY FOR BIOLOGICALLY ACTIVE HUMAN INTERFERON-GAMMA EMPLOYING UNIQUE MONOCLONAL ANTIBODIES Abstract Rapid, sensitive and accurate immunoassays for biologically active natural or recombinant human interferon-gamma (huIFN-gamma) based upon monoclonal antibodies which react specifically with epitopes of the biologically active form of huIFN-gamma are disclosed. The immunoassays include sandwich immunoradiometric assay of the forward, reverse or simultaneous type and competitive binding assay such as radioimmunoassay. The assays are also useful for the detection of macrophage activation factor now believed to be identical to huIFN-gamma. In addi- tion, methods of purification of huIFN-gamma employ- ing the monoclonal antibodies are described.

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