C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
167/37, 195/1.10
C12N 5/02 (2006.01) C07K 16/10 (2006.01) C07K 16/30 (2006.01) G01N 33/577 (2006.01)
Patent
CA 1248471
IMMUNOASSAY FOR BREAST CANCER EMPLOYING MONOCLONAL ANTIBODIES ABSTRACT OF THE DISCLOSURE An immunoassay for detecting human breast cancer includes a monoclonal antibody directed to viral related protein derived from the T47D clone-10 breast cancer cell line. This monoclonal antibody is from hybridoma cell line ATCC No. HB 8630. In one preferred embodiment, an immunoassay for human breast cancer in a tissue sample includes incubating the sample with the monoclonal antibody and detecting the formation of an antibody-antigen complex using the indirect immunoperoxidase assay or radioimmune assay involving an antibody directed to the monoclonal antibody. Another preferred embodiment for detecting human breast cancer involves contacting a fluid sample, such as blood serum, with two antibodies, one of which is soluble and one of which is fixed to a solid substrate. One of the two antibodies is the monoclonal antibody of the present invention specific for human mammary tutor virus, and one of the antibodies is detectable. The formation of the antibody antigen complex can be detected using the radioimmune assay, or enzyme linked immunosorbent assay techniques.
488983
Gowling Lafleur Henderson Llp
Tel Aviv University
Teva Pharmaceutical Industries Ltd.
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