Immunoassay for cellular proteins

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1333149

An immunoassay for cellular proteins which may be present as contaminants in a product purified from mammalian cell culture. Since mammalian cell culture requires the use of protein-containing media, the assay must be specific in recognizing cellular proteins but not media proteins. This is accomplished by selective adsorption of an antiserum to cellular proteins against media proteins. Quantification of the assay may be improved through the use of a purified antigen, namely fibronectin, which is known to be highly immunogenic.

614477

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