Immunoassay for class specific immunoglobulin antibodies

Details Immunoassay for class specific immunoglobulin antibodies Immunoassay for class specific immunoglobulin antibodies

G - Physics

01

N

167/45

G01N 33/543 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1215644

ABSTRACT The present invention relates to a method for determining an immunoglobulin antibody of an IgX class in a sample, wherein X is M, A, D or E, comprising: a) treating the sample with an effective amount of anti- IgG; b) contacting the treated sample with a class specific antibody reagent comprising an antigen, for which the IgX antibody is specific, coated on a solid support, to form an antigen-IgX complex on the solid support; c) removing unbound sample; d) treating the antigen-IgX complex with anti-IgX; and e) determining the anti-IgX bound to the antigen-IgX complex as a measure of the IgX in the sample. The method provides advantages over prior art methods, including overcoming rheumatoid factor interference in the determination.

418218

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