Immunoassay for measuring human c-peptide and kit therefor

Details Immunoassay for measuring human c-peptide and kit therefor Immunoassay for measuring human c-peptide and kit therefor

G - Physics

01

N

G01N 33/74 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2348379

An immunoassay for selectively measuring human C-peptide as well as a kit therefor is disclosed. In the method, human C-peptide contained in a sample, a first anti-human C-peptide antibody, and a second anti-human C-peptide antibody which is immobilized on a solid support are reacted to form an immune complex among these three components. The formed immune complex is separated from the non-reacted antibodies and sample; and then the separated immune complex is quantified. The first antibody recognizes an epitope existing in the region from 1st to 10th amino acid residue from the N-terminal of the human C-peptide, and the second antibody recognizes an epitope existing in the region from 1st to 16th amino acid residue from the N-terminal of human C-peptide; with the proviso that the first and second antibodies do not recognize the same epitope so that they can simultaneously bind to said human C-peptide.

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