Immunoassay for mycophenolic acid

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/44 (2006.01) C07D 307/88 (2006.01) C07K 2/00 (2006.01) C12N 9/96 (2006.01) G01N 33/53 (2006.01) G01N 33/577 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2194439

The present invention provides antibodies useful in assays for mycophenolic acid (MPA). These antibodies bind MPA and are able to distinguish MPA from its esters, such as morpholineothyl E-6-(1,3-dihydro-4-hydroxy-6-methoxy-7-methyl- 3-oxo-5-isobenzofuranyl)-4-methyl-4-hexenoate, and/or its metabolites, such as mycophenolic acid glucuronide. The present invention also provides conjugates of labels and MPA or MPA analogs. The antibodies of the invention are capable of binding these conjugates and are also capable of inhibiting the activity of the label when bound to the conjugates. The present invention also provides methods for the determination of MPA in a sample suspected of containing MPA that use the antibodies and/or conjugates of the invention. The present invention also provides assay reagents as well as packaged kits useful for performing the methods of the invention.

La présente invention concerne des anticorps utilisés dans des dosages de l'acide mycophénologique (AMP). Ces anticorps fixent l'AMP et peuvent faire la distinction entre l'AMP et ses esters, tel que le morpholinoéthyle E-6-(1,3-dihydro-4-hydroxy-6-méthoxy-7-méthyl-3-oxo-5-isobenzofuranyl)-4-méthyl-4-hexénoate, et/ou ses métabolites, tels que l'acide glucuromique mycophénolique. La présente invention permet d'obtenir des conjugués de marques et d'AMP ou d'analogues de l'AMP. Les anticorps selon l'invention peuvent fixer ces conjugués et peuvent également inhiber l'activité de la marque lorsqu'ils sont liés aux conjugués. La présente invention se rapporte par ailleurs à des procédés de détermination de l'AMP dans un échantillon susceptible de contenir de l'AMP à l'aide des anticorps et/ou des conjugués de l'invention. La présente invention permet également d'obtenir des réactifs de dosage ainsi que des kits conditionnés utilisés pour mettre en oeuvre les procédés de l'invention.

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