C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/26 (2006.01) G01N 33/564 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2683911
The present invention relates to an immunoassay for detection of BNP, proBNP and fragments thereof. Essentially the assay comprises: a) contacting the antigen with a first antibody specific to a fragment corresponding to amino acids 11-22 of BNP, or to a part of this peptide comprising at least three amino acids of said sequence, to obtain a first order immune complex. b) contacting the first order immune complex obtained at step (a) with a second antibody recognizing said first order immune complex, to obtain a second order immune complex, wherein said antibody is unable to recognize free BNP, proBNP or free first antibody; c) Detecting the second order immune complex.
La présente invention concerne un immuno-dosage pour détecter BPN, proBNP et des fragments de ceux-ci. Le dosage consiste essentiellement : à mettre en contact un antigène avec un premier anticorps spécifique d'un fragment correspondant aux acides aminés 11 à 22 de BNP, ou à une partie de peptide comprenant au moins trois acides aminés de la séquence pour obtenir un complexe immun de premier ordre; b) à mettre en contact le complexe immun de premier ordre obtenu à l'étape (a) avec un second anticorps qui reconnaît le complexe immun de premier ordre pour obtenir un complexe immun de second ordre, ledit anticorps n'étant pas capable de reconnaître BNP libre, proBNP libre ou un premier anticorps libre; c) et à détecter le complexe immun de second ordre.
Filatov Vladimir L.
Katrukha Alexey G.
Kolosova Olga V.
Tamm Natalia N.
Hytest Ltd.
Marks & Clerk
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