Immunoassay for the dectection of human autoantibodies

G - Physics – 01 – N

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G01N 33/576 (2006.01) G01N 33/543 (2006.01) G01N 33/564 (2006.01)

Patent

CA 2175216

An assay and test kit for the determina- tion of LKM-1 autoantibodies in test samples suspected of containing anti-LKM-1 autoanti- bodies. The method uses a solid phase which preferably is a microparticle. The method is standardized and can be performed in auto- mated systems, allowing quantitation of the amount of anti-LKM antibody in test samples.

Un immunotitrage et une trousse d'immunotitrage permettent de détecter la présence d'autoanticorps LKM-1 (autoanticorps microsomiques du foie et du rein) dans des échantillons soupçonnés de contenir des autoanticorps anti-LKM-1. Le procédé utilisé recourt à une phase solide constituée de préférence d'une microparticule. Ce procédé est normalisé, peut être effectué dans des systèmes automatiques et permet de quantifier des anticorps anti-LKM dans des échantillons analysés.

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