Immunoassay method for detecting antibodies to antigens

G - Physics – 01 – N

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167/44

G01N 33/543 (2006.01)

Patent

CA 1300500

- 1 - IMMUMOASSAY METHOD FOR DETECTING ANTIBODIES TO ANTIGENS Case Docket No. 4840P APPLICANTS: William J. Todd Paul A. Barstad ABSTRACT OF THE DISCLOSURE An immunoassay method for the detection in one- step of specific antibodies. The method includes incubation of a solid phase support or matrix having a spot of the antigens component bound thereto with a a sample of the clinical fluid containing the anti- bodies component in the presence of a signal developing reagent, including a detector substance, which is pre- ferably a colloidal metal sol, and a ligand, such as protein A or other antibody binding ligand. A diag- nostic field kit containing the test antigens and signal developing reagent is also described. - 1 -

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