Immunoassay methods using noncross reactive cea gene family...

Details Immunoassay methods using noncross reactive cea gene family... Immunoassay methods using noncross reactive cea gene family...

C - Chemistry, Metallurgy

12

P

167/37, 195/1.10

C12P 21/08 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2036237

ABSTRACT Immunoassay methods useful for the determination of the presence or absence of CEA gene family members are provided. Methods are provided that differentiate between members of the CEA gene family. In addition, monoclonal antibodies against CEA-family members, said antibodies specific to TM-CEA, CEA, or NCA, and not cross-reactive with other CEA family members are provided. Further, antibodies specific to two of three CEA gene family member, TM-CEA and NCA but not CEA and CEA and NCA but not TM-CEA, TM-CEA and CEA but not NCA, are provided. Said monoclonal antibodies include antibodies against native protein and antibodies against denatured protein. Also provided are hybridoma cell lines secreting said antibodies, and pharmaceutical compositions containing said antibodies or fragments thereof.

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