Immunoassay plate and use thereof

G - Physics – 01 – N

Patent

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Details

G01N 33/545 (2006.01) B01L 3/00 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2161301

An immunoassay plate for an immune complex transfer immunoassay, comprising a well type solid phase and a dip stick type solid phase which can be inserted into said well type solid phase, wherein the dip stick type solid phase is coated with either substance (A) or (B) to be mentioned below and the well type solid phase is coated with the other, remaining substance, and these solid phases are used as the two solid phases to be used for an immune complex transfer immunoassay: (A): a substance having a reactive group which specifically binds to a functional group previously introduced onto a substance, which specifically forms an immune complex with a test substance (B): a substance having a reactive group capable of specifically binding to the test substance in the immune complex, a substance which specifically forms an immune complex with the test substance, or a functional group conjugated in advance with said substance, provided that the moiety which binds to the reactive group of (A) does not bind to the reactive group of (B) and vice versa. According to the present invention, an immune complex transfer immunoassay can be markedly simplified. Consequently, a highly sensitive immune complex transfer immunoassay can be conducted with ease and with high precision.

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