Immunoassay utilizing biotin bridge with universal solid phase

Details Immunoassay utilizing biotin bridge with universal solid phase Immunoassay utilizing biotin bridge with universal solid phase

G - Physics

01

N

167/44

G01N 33/74 (2006.01) G01N 33/532 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1333883

An immunoassay utilizing a biotin bridge is performed with a solid phase with anti-biotin immobilized on its surface. In accordance with the assay, the solid phase is incubated with a biotinylated binding species, preferably biotinylated antibody, specific for the ligand to be detected. This is then reacted with the test sample, followed by a labeled binding member, either in a sandwich type or competitive-type binding assay. The label is then detected in accordance with conventional detection techniques. Use of the solid phase with only a biotin-specific antibody attached thereto expands the utility and versatility of a single solid phase for use in the detection of an unlimited variety of ligands.

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