Immunoassays using a carbon sol label

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12Q 1/25 (2006.01) C12Q 1/56 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/532 (2006.01) G01N 33/543 (2006.01) G01N 33/551 (2006.01) G01N 33/561 (2006.01) G01N 33/58 (2006.01) G01N 33/76 (2006.01) G01N 33/78 (2006.01)

Patent

CA 2149062

Method for determining the presence or amount of an analyte in a sample by contacting the sample with a carbon-labeled constituent comprising an essentially non-stabilized aqueous sol of a carbon black having a linear predictor value V > 0, wherein V = - 138.954 - 0.987xDBP + 15.609xVC + 3.994xPPD; DBP is the dibutylphthalate adsorption in ml/100g, as determined according to DIN 53601; VC is the volatile content in %, as determined according to DIN 53552; and PPD is the average primary particle diameter in nanometers; the colloidal carbon particles having directly conjugated to their surface a binding component capable of specifically recognizing said analyte and determining the presence or absence of a resulting analyte/carbon particle complex as an indication of the presence or a measure of the amount of analyte in said sample. Composition suited for use in the method, and method for preparing same.

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