Immunochemical assay of human chorionic gonadotropin and...

C - Chemistry – Metallurgy – 07 – F

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C07F 7/00 (2006.01) C12Q 1/28 (2006.01) G01N 33/76 (2006.01)

Patent

CA 1218300

Abstract of the Disclosure A immunochemical assay of human chorionic gonadotropin (hCG) involves the use of an antibody supported on a carrier, an antigen and an antibody labelled with peroxidase, wherein the supported antibody and the labeled antibody are dissimilar and are not overlapping with each other in antigen-determinant position, the supported antibody is specifically reactive to hCG, the peroxidase is coupled with the corresponding antibody with a compound of the formula: Image (I) wherein n is an integer of 0 to 5 and R is a chemical bond or a divalent 6-membered cyclic hydrocarbon residue, and a high reproducibility of measurement of hCG is obtained.

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