Immunochemical assay of human chorionic gonadotropin and...

G - Physics – 01 – N

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G01N 33/76 (2006.01) G01N 33/535 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1196280

ABSTRACT OF THE DISCLOSURE The immunochemical assay of human chorionic gonadotropin (hCG) is disclosed involving the use of an antibody supported on a carrier, an antigen and an antibody labeled with a labeling agent. The supported antibody and the labeled antibody are dissimilar antibodies which are non-overlapping with each other in antigen-determinant position, the supported antibody is an antibody specifically reactive to hCG, and the labeling agent is .beta.-D-galactosidase which is coupled with the corresponding antibody using N,N'-O-phenylene-dimaleimide. The method enables a high reproducibility to hCG to be obtained.

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