Immunochemical detection of human uterine endometrial cancer...

G - Physics – 01 – N

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G01N 33/577 (2006.01) G01N 33/567 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2063652

ABSTRACT OF THE DISCLOSURE A process has been developed by which any one can conveniently, objectively, and accurately detect uterine endometrial cancer cells without requiring any special technique or equipment. The process comprises the steps of preparing a specimen by collecting endometrial cells and/or endometrial tissue from cavity of uterus, and optionally disrupting the collected cells and/or the tissue, immobilizing the specimen on two or more reaction areas on one or more carrier, allowing a labelled monoclonal antibody MSN-1 prepared by binding a labelling agent to MSN-1 antibody which specifically reacts with the uterine endometrial cancer cell, and a labelled anti-acitin antibody prepared by binding a labelling agent to an anti-actin antibody which is reactive to actin which is generally present in human cells to react with the immobilized specimen, respectively, removing the labelled MSN-1 antibody and the labelled anti-actin antibody which have failed to bind to the specimen, measuring signal intensities y and x of the labelling agents of the labelled MSN-1 antibody and the labelled anti-actin antibody, which have been bound to the specimen, respectively, and determining presence or absence of the uterine endometrial cancer cell in the specimen by evaluating the signal intensity y indicated by the labelling agent of the labelled MSN-1 antibody in relation to the signal intensity x indicated by the labelling agent of the labelled anti-actin antibody. The detection may also be carried out by using the MSN-1 antibody and the anti-actin antibody together with a labelled-secondary antibody.

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