Immunochemical ldh.sub.1 assay

Details Immunochemical ldh.sub.1 assay Immunochemical ldh.sub.1 assay

C - Chemistry, Metallurgy

12

Q

167/44

C12Q 1/00 (2006.01) G01N 33/573 (2006.01)

Patent

CA 1117414

RAN 4093/38 ABSTRACT LDH1 levels in serum samples can be rapidly and accurately assayed by a novel immunochemical technique. In such procedure the serum sample is treated with soluble antibodies against the M subunit and the resulting antigen-antibody reaction product is insolubilized with a second antibody supported on an insoluble material. The resulting supernatant containing only LDH, isoenzyme is assayed for enzymatic activity by conventional procedures. The assay of LDH1 levels in sera is useful in determining whether the subject has undergone a myocardial infarction.

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