Immunochemicals bound to labelled particles and use thereof

Details Immunochemicals bound to labelled particles and use thereof Immunochemicals bound to labelled particles and use thereof

G - Physics

01

N

167/41, 167/43

G01N 23/00 (2006.01)

Patent

CA 1038290

ABSTRACT OF THE DISCLOSURE A broad new class of reagents permits extremely sensitive and specific assay for, or chemical separation of , a broad range of biological and nonbiological substances. Each reagent consists of a suspension of microscopic carrier material particles, each particle bearing (1) tracer material-- fluorescent, radioactive or otherwise -- and (2) a coating of biological homologue, that is, antibody, antigen, or portions or equivalents thereof, for the substance whose assay is desired. The latter substance if introduced into the suspension links the particles together in pairs or clumps, which may be sensitively and accurately detected by monitoring the tracer. The carrier is preferably partially hydrolyzed polyacrylamide resin, or in appropriate applica- tions acrylic acid and other derivatives thereof, and other polymers including agar, and the coupling effected by covalent bonding. Other embodiments, including various mechanical forms of carrier, for greater ease of handling and separation, are also described.

200072

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