C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/08 (2006.01) A61K 39/00 (2006.01) C07K 16/44 (2006.01) C12Q 1/68 (2006.01) C12Q 1/70 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01)
Patent
CA 2129163
The present invention relates to a monoclonal antibody 20D3 which specifically recognizes the RNA:DNA hybrid (HNA) and does not cross-react with single- stranded or double-stranded DNA or RNA. The present invention also relates to an ELISA method for the immunodetection of DNA:RNA hybrid (HNA) to indicate the absence or presence of a pathogen in a sample, which comprises the steps of: a) incubating said sample with at least one DNA probe specific to said pathogen RNA for a time sufficient for HNA formation to occur; b) incubating said HNA of step a) with the monoclonal antibody of the present invention for antibody-HNA duplex to occur; c) detecting said antibody-HNA duplex of step b); thereby determining the presence of said pathogen in said sample, such as a Listeria sp. or an oncogene expressed by a tumor.
Emond Eric
Fliss Ismail
Lemieux Real
Pandian Sithian
Simard Ronald E.
Lemieux Real
Swabey Ogilvy Renault
Universite Laval
LandOfFree
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