Immunogenic compostion and method of developing a vaccine...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/00 (2006.01) A61K 39/00 (2006.01) C12P 21/04 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2541506

An antigenic and immunogenic composition of predetermined inactivated strains of human immunodeficiency virus (HIV) is disclosed. Inactivation is through psoralen and ultraviolet radiation; the composition is rendered more effective by the removal of structural features of HIV that interfere with immune response. In particular, sialic acid is removed to enhance immune recognition of the composition and to impair Complement Factor H binding. CD55 and CD59 are also removed to prevent the binding of Complement Factor H. Determination of strains for inactivation may be though immunotherapeutic genotyping or probabilistic assessment of risk of exposure.

L'invention concerne une composition antigénique et immunogène comprenant des souches inactivées prédéterminées du virus de l'immunodéficience humaine (VIH). L'inactivation desdites souches est réalisée au moyen de psoralène et par exposition à un rayonnement ultraviolet. La composition selon l'invention est rendue plus efficace par suppression de caractéristiques structurales du VIH qui interfèrent avec la réponse immunitaire. En particulier, l'acide sialique est supprimé pour renforcer la reconnaissance immunitaire de la composition et pour affecter la liaison du facteur H du complément. Les molécules CD55 et CD59 sont également supprimées pour empêcher la liaison du facteur H du complément. Les souches destinées à être inactivées peuvent être déterminées par génotypage immunothérapeutique ou évaluation probabiliste du risque d'exposition.

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