Immunogenic hiv-1 multi-clade, multivalent constructs and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/49 (2006.01) A61K 39/21 (2006.01) A61K 48/00 (2006.01) C07K 14/16 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2519025

Described herein are nucleic acid molecules which encode multiple highly conserved epitopes from HIV-1 proteins, and optionally also epitopes from CCR5; usually also included sequences that encode spacers between two or more of the epitopes. Some of the provided nucleic acid molecules further include sequences that encode targeting domains, useful for targeting the encoded protein into a pathway for enhancing epitope presentation in a vertebrate immune system. Also described are multivalent proteins encoded for by these nucleic acid molecules. The disclosure also encompasses immunogenic compositions that comprise one or more of the nucleic acid molecules, and/or one or more of the proteins encoded thereby, as well as methods of inducing an immune response against HIV-1 in a subject by administering to the subject an effective amount of a composition containing one or more of these molecules. Also provided are cultured host cells containing within them one or more of the described nucleic acid molecules.

L'invention concerne des molécules d'acide nucléique codant pour des épitopes multiples hautement conservées de protéines HIV-1, et pouvant comprendre éventuellement des épitopes de CCR5, et comprenant en général en outre des séquences incluses codant pour des espaceurs entre au moins deux épitopes. Certaines des molécules d'acide nucléique décrites contiennent en outre des séquences codant pour des domaines de ciblage, et permettant le ciblage de la protéine codée sur une voie favorisant la présentation de l'épitope dans le système immunitaire d'un vertébré. L'invention concerne également des protéines multivalentes codées par ces molécules d'acide nucléique, et des compositions immunogènes qui contiennent une ou plusieurs de ces molécules d'acide nucléique, et/ou une ou plusieurs des protéines codées par ces dernières, ainsi que des méthodes permettant d'induire une réponse immunitaire contre le VIH-1 chez un sujet en lui administrant une dose efficace d'une composition contenant une ou plusieurs de ces molécules. L'invention concerne en outre des cellules hôtes issues de cultures, contenant une ou plusieurs des molécules d'acide nucléique décrites.

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