Immunoglobulin frameworks which demonstrate enhanced...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) C07K 16/00 (2006.01) C07K 16/18 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2483285

Compositions are provided, which can be used as frameworks for the creation of very stable and soluble single-chain Fv antibody fragments. These frameworks have been selected for intracellular performance and are thus ideally suited for the creation of scFv antibody fragments or scFv antibody libraries for applications where stability and solubility are limiting factors for the performance of antibody fragments, such as in the reducing environment of a cell. Such frameworks can also be used to identify highly conserved residues and consensus sequences which demonstrate enhanced solubility and stability.

La présente invention concerne des compositions pouvant servir d'infrastructures pour la création de fragments très stables et solubles d'anticorps Fv monocaténaires. Ces infrastructures, qui ont été sélectionnées pour leur comportement intracellulaire, conviennent particulièrement pour la création de fragments d'anticorps scFv ou d'échantillothèques d'anticorps scFv, notamment dans le cas d'applications où la stabilité et la solubilité sont des facteurs limitants affectant le comportement de fragments d'anticorps, notamment dans l'environnement réducteur d'une cellule. De tels fragments conviennent également pour identifier des résidus hautement conservés et des séquences consensus faisant preuve de solubilité et stabilité renforcées.

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