Immunohistochemical methods for determining signal...

G - Physics – 01 – N

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G01N 33/50 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2650776

The method of the invention pertains to determining the signal transduction activity in a tissue section by immunohistochemistry techniques. The expression level of the receptor of interest is determined as well as the expression levels of one or more effector molecules of the receptor signal transduction pathway. Furthermore a combined ratio of expression levels of effector molecules in subcellular compartments with the receptor expression was found to have prognostic significance.

L'invention concerne un procédé de détermination de l'activité de transduction de signal dans une section de tissu selon des techniques d'immunohistochimie. Le niveau d'expression du récepteur présentant un intérêt est déterminé, ainsi que les niveaux d'expression d'une ou de plusieurs molécules effectrices du processus de transduction du signal récepteur. En outre, un rapport combiné de niveaux d'expression de molécules effectrices dans des compartiments subcellulaires avec l'expression réceptrice s'est avéré présenter une importance sur le plan pronostic.

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