Immunohistochemical staining composition

G - Physics – 01 – N

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G01N 33/531 (2006.01) G01N 1/30 (2006.01) G01N 33/574 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2266405

An immunohistochemical staining composition characterized by containing a diagnostic marker containing an antibody having a fluorescent functional group and a substance selected from the group consisting of glycerophospholipids, fatty acids and sugar derivatives serving as a surfactant. The composition is excellent in fluorescence intensity and is free from the fear of damages to biological tissues or DNAs caused by ultraviolet excitation, thus being useful for biological immunohistochemical staining. The composition makes it possible to efficiently and safely conduct, for example, the quasi-intracorporeal early diagnosis of malignant neoplasm of the epithelial tissues, such as esophageal carcinoma, gastric cancer or colon cancer, with an infrared endoscope and the specification and diagnosis of foci in a surgical operation.

La présente invention concerne une composition de coloration immunohistochimique dont la caractéristique est de contenir un anticorps portant un groupe fonctionnel fluorescent et une substance appartenant au groupe des glycérophospholipides, des acides gras et des dérivés du sucre, et intervenant comme agent tensio-actif. Cette composition, qui fait preuve d'une très bonne intensité de fluorescence, ne présente pour les tissus biologiques ou les ADN aucun risque imputable à l'excitation par l'ultraviolet, ce qui fait qu'elle est particulièrement adaptée à la coloration immunohistochimique des agents biologiques. Cette composition permet, au moyen d'un endoscope à infrarouge, de mener à bien avec efficacité et en toute sécurité le diagnostic précoce quasi-intracorporel des tumeurs malignes épithéliales telles que le cancer de l'oesophage, le cancer de l'estomac ou le cancer du colon. Cette composition permet également de désigner et de diagnostiquer les foyers dans une opération chirurgicale.

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