G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/552 (2006.01) G01N 33/532 (2006.01) G01N 33/544 (2006.01) G01N 33/545 (2006.01) G01N 33/58 (2006.01)
Patent
CA 1264662
Abstract of the Disclosure This invention is directed to a new membrane lytic immunoassay. In one embodiment of this assay, an antigen is first covalently coupled with lipids and this antigen-lipid complex is mixed with a hexagonal phase forming lipid to form bilayer liposome vesicles additionally containing a self-quenching fluorescent dye. When this antigen-containing liposome is brought into contact with a solid surface coated with antibody molecules, binding occurs between the antigen and the antibody, disrupting the liposome and releasing the dye. To assay a biological fluid for free antigen the fluid is first contacted with the solid surface-antibody complex, to saturate the bound antibody. Binding by the liposomes is thereby inhibited, leading to reduced dye release. Comparing dye release against a standardized curve of dye release versus known antigen concentrations allows for rapid determination of the unknown antigen concentration in the biological fluid. Similarly, antibodies and other entities, e.g., enzymes, drugs, etc., may be determined using slightly modified versions of this assay.
483617
Ho Rodney Jin Yong
Huang Leaf
Swabey Ogilvy Renault
The University Of Tennessee Research Corporation
LandOfFree
Immunoliposome assay - methods and products does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Immunoliposome assay - methods and products, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Immunoliposome assay - methods and products will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1261586