Immunological assay for plasmin-digested products of...

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G01N 33/53 (2006.01) G01N 21/76 (2006.01) G01N 21/78 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2606169

A method of immunologically analyzing a plasmin degradation product of stabilized fibrin characterized in that a) a monoclonal antibody that does not react with stabilized fibrin, fibrinogen or a plasmin degradation product of fibrinogen but reacts with a neoantigen of D domain generated by plasmin degradation of stabilized fibrin and (b) a monoclonal antibody that specifically reacts with a plasmin degradation product of stabilized fibrin by recognizing a site different from that for the monoclonal antibody (a) are used in combination, one of the monoclonal antibodies (a) and (b) is conjugated to a magnetic particle and the other monoclonal antibody is labeled with an enzyme, and a chemiluminescent substrate is used as a substrate for the enzyme is disclosed.

L'invention porte sur une méthode pour analyser immunologiquement un produit de dégradation de la plasmine de fibrine stabilisée, caractérisée en ce que a) un anticorps monoclonal qui ne réagit pas avec la fibrine stabilisée, le fibrinogène ou un produit de dégradation de la plasmine du fibrinogène, mais réagit avec un néoantigène de domaine D produit par dégradation de la plasmine de fibrine stabilisée et (b) un anticorps monoclonal qui réagit spécifiquement avec un produit de dégradation de la plasmine de fibrine stabilisée en reconnaissant un site différent de celui de l'anticorps monoclonal (a) sont utilisés en combinaison ; un des anticorps monoclonaux (a) et (b) est conjugué à une particule magnétique et l'autre anticorps monoclonal est marqué avec une enzyme, et un substrat chimioluminescent est utilisé comme substrat pour l'enzyme.

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