Immunological detection of rna:dna hybrids on microarrays

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 31/22 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) G01N 37/00 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2391558

The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably labeled complementary biomolecule to form a double- stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.

La présente invention concerne une méthode d'hybridation et un kit de détection et de mesure de molécules biologiques. Un échantillon d'essai contenant les molécules biologiques d'intérêt est hybridé avec une biomolécule non marquée ou facultativement avec une biomolécule complémentaire marquée décelable en vue de former un hybride à brin double immobilisé à une phase solide. L'hybride immobilisé peut être détecté au moyen d'une entité qui reconnaît spécifiquement un hybride d'ARN:ADN, étape suivie par des analyses et une quantification. Pour ce faire, la présente invention concerne une méthode et un kit pour détecter et mesurer des molécules biologiques, simples à utiliser, hautement spécifiques, sensibles et précis lors du criblage de plusieurs molécules biologiques.

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