Immunological determinations

Details Immunological determinations Immunological determinations

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/538 (2006.01) G01N 33/552 (2006.01)

Patent

CA 2224157

A method of effecting an immunological determination of an analyte in a fluid sample, the method comprises the steps of (i) providing a column of a solid support material having a first binding agent which is covalently linked to the solid support material and which is capable of forming an immunological complex with said analyte, (ii) introducing the fluid sample onto the column to allow analyte (if present) therein to form an immunological complex with said first binding agent; (iii) providing a label bound to the immunological complex formed under (ii), and (iv) using the immobilised label in situ on the support to determine the analyte.

Méthode d'immunodosage d'un analyte dans un échantillon liquide, la méthode comprenant les étapes suivantes : i) fournir une colonne constituée d'une matière servant de support solide possédant un premier agent liant qui est lié par covalence au support solide et qui est capable de former un complexe immun avec ledit analyte, ii) introduire l'échantillon liquide dans la colonne pour laisser l'analyte (s'il est présent) former un complexe immun avec ledit premier agent liant, iii) fournir un marqueur lié au complexe immun formé en ii), et iv) utiliser le marqueur immobilisé in situ sur le support pour doser l'analyte.

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