Immunomagnetic capture and imaging of biological targets

G - Physics – 01 – N

Patent

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Details

G01N 33/543 (2006.01) G01N 15/00 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2711564

The present invention relates to methods and systems for labeling, isolating, detecting, and/or enumerating a statistically significant number of biological cells, or other biological analytes of interest, present in a complex matrix sample. The isolation of a biological target of interest from a sample mixture is done by immunomagnetic separation. Upon introduction of the sample within an imaging chamber, the capture complex (biological target- magnetic capture agent) will be attracted by the magnetic field and will lay on the surface of the chamber in the focal plane of the imaging system.

L'invention concerne des procédés et des systèmes pour marquer, isoler, détecter et/ou dénombrer un nombre statistiquement significatif de cellules biologiques ou d'autres analytes biologiques d'intérêt, présents dans un échantillon de matrice complexe. L'isolement d'une cible biologique d'intérêt d'un mélange d'échantillons est effectué par une séparation immunomagnétique. Lors de l'introduction de l'échantillon dans une chambre d'imagerie, le complexe de capture (cible biologique-agent de capture magnétique) sera attiré par le champ magnétique et se trouvera sur la surface de la chambre dans le plan focal du système d'imagerie.

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