Immunometric determination of an antigen or hapten

Details Immunometric determination of an antigen or hapten Immunometric determination of an antigen or hapten

G - Physics

01

N

G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01) G01N 33/74 (2006.01) G01N 33/88 (2006.01)

Patent

CA 2113383

The invention relates to a process for the immunometric determination of an antigen or hapten. According to this process, contacting takes place (fig. 1A) between the antigen or hapten (3) to be determined and the antibodies (2) fixed to a solid phase in order to immunologically bond the antigen or hapten with the antibody. This is followed (fig. 1B) by immobilizing the antigen or hapten (3) by a covalent bond (4) to the solid phase (1) whilst releasing its epitope (fig. 1C). This is followed by the contacting thereof (fig. 1D) with labelled antibodies (5) and determination takes place (fig. 1E) of the quantity of fixed labelled antibodies in order to deduce therefrom the initial hapten or antigen concentration. As a result of this stage of immobilizing and releasing the epitope, a high sensitivity is obtained using a single antibody.

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