Immunomodulatory oligonucleotides

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/39 (2006.01) A61K 31/7088 (2006.01) A61P 31/00 (2006.01) A61P 31/12 (2006.01) A61P 35/00 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2194761

Oligonucleotides containing unmethylated CpG dinucleotides and therapeutic utilities based on their ability to stimulate an immune response in a subject are disclosed. Also disclosed are therapies for treating diseases associated with immune system activation that are initiated by unmethylated CpG dinucleotides in a subject comprising administering to the subject oligonucleotides that do not contain unmethylated CpG sequences (i.e. methylated CpG sequences or no CpG sequence) to outcompete unmethylated CpG nucleic acids for binding. Further disclosed are methylated CpG containing dinucleotides for use in antisense therapies or as in vivo hybridization probes, and immunoinhibitory oligonucleotides for use as antiviral therapeutics.

On décrit des oligonucléotides contenant des dinucléotides à CpG non méthylé ainsi que des applications thérapeutiques basées sur l'aptitude de ces oligonucléotides à stimuler une réponse immune chez un sujet. On décrit également des thérapies destinées à traiter des maladies associées à l'activation du système immun et qui sont amorcées chez un sujet par des dinucléotides à CpG non méthylé, ces thérapies consistant à administrer audit sujet des oligonucléotides ne contenant pas de séquence à CpG non méthylé (c'est-à-dire des séquences à motif CpG méthylé ou pas de séquence à motif CpG) afin de mettre hors concours les acides nucléiques à CpG non méthylé aux fins de liaison. On décrit en outre des dinucléotides contenant CpG méthylé, utiles dans des thérapies antisens ou en tant que sondes d'hybridation in vivo, ainsi que des oligonucléotides immuno-inhibiteurs utiles en tant que thérapeutiques antivirales.

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