Immunostimulatory dna

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) A61K 31/70 (2006.01) C12N 15/11 (2006.01) G01N 33/566 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2352084

Methods of identifying immunostimulatory DNA are provided, which methods include (i) detection of immunostimulatory DNA by formation of a complex between the DNA and isolated Replication Protein A (RPA) subunits; and (ii) down-regulation of CSF-1R expression by macrophages or macrophage cell cycle arrest. Also provided are immunostimulatory DNA agonists and antagonists.

L'invention se rapporte à des méthodes d'identification d'ADN immunostimulateur, qui consistent (i) à détecter l'ADN immunostimulateur par formation d'un complexe entre l'ADN et des sous-unités isolées de protéine de réplication A (RPA); et (ii) à procéder à une régulation négative de l'expression de CSF-1R par les macrophages ou à l'arrêt du cycle cellulaire des macrophages. L'invention se rapporte également à des agonistes et antagonistes de l'ADN immunostimulateur.

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